MODELING OF LIGHT DISTRIBUTION IN HUMAN CERVIX TISSUES
FOR PHOTODYNAMIC THERANOSTIC APPLICATIONS

Nedret Erol1, Necla Kenar1

1Department of Physics, Kocaeli University, 41380 Umuttepe, Kocaeli TURKEY

ABSTRACT
We aimed in this study to provide light propagations in 1D, 2D, and 3D in human tissues having different geometries for different wavelengths located Photodynamic Theranostic window to show that tissue is cancerous or not and treat. Photon transport is investigated according time, position and angle using Diffusion Equation with Perturbation Theory and Monte Carlo Simulations. Optical properties (the absorption coefficient (μa)) , the scattering coefficient (μs), refractive index (n), anisotropy factor (g) used in these calculations obtained from literature. Time-dependent and steady-state reflectance, transmittance, absorbance, penetration depths and fluence values are modelled. All models are studied with photosensitizier (PS) and PS free for continuous wave (CW) light sources. It was observed that the scattering perturbations and penetration of CIN 2 and CIN 3 tissues were almost the same as that of cervical tissue modeling of light-tissue interactions for semi-infinite geometry using the optical properties obtained from the literature, while scattering perturbation of normal tissue was less than CIN 2 and CIN 3, CIN III showed lower light absorption than normal cervical tissue. Between the four wavelengths, normal tissue, homogeneous in CIN2 and CIN3 tissues, and scattering perturbation and penetration values were observed to be maximal at the wavelength of 811 nm. In the Absorption Perturbation, the wavelength was measured as 956 nm, which has the highest absorption perturbation in the tissues.

 

OPTICAL CHARACTERIZATION OF HUMAN TISSUES FOR PHOTODYNAMIC THERANOSTIC APPLICATIONS

Nedret Erol1, Necla Kenar1, Hyun Soo Lim2

1Department of Physics, Kocaeli University, 41380 Umuttepe, Kocaeli TURKEY

2 Sakarya University, Faculty of Technology, Department of Electrical and Electronics Engineering

We aimed in this study to provide light propagations in 1D, 2D, and 3D in human tissues having different geometries for different wavelengths located Photodynamic Theranostic window  to show that tissue is cancerous or not and treat. Photon transport is investigated according time, position and angle using Diffusion Equation with Perturbation Theory and Monte Carlo Simulations. Optical properties( the absorption coefficient (µa), the scattering coefficient(µs), refractive index(n), anisotropy factor (g)) used in these calculations obtained from literature. Time-dependent and steady-state reflectance, transmittance, absorbance, penetration depths and fluence values are modeled. All models are studied with photosensitizier(PS) and PS free for continuous wave(CW) light sources.

TFD 2017 Bodrum , TURKEY

 

BIOINFORMATIC TOOLS IN THE STUDY OF MOLECULAR EVOLUTION

Nedret EROL1, Paolo Mereu2

1Department of Physics, Kocaeli University, 41380 Umuttepe, Kocaeli TURKEY

2Department of Biomedical Science, Sassari University, Italy

Basic knowledge of the main steps requested to infer the phylogeny of a species and to estimate the genetic diversity intra- and inter-populations.

Analysis of nucleotide sequencing raw data, DNA sequences characterization and detection of functional domains at the protein level. Search for homologous sequences in the GenBank database by means of the BLAST tool. Comparative analysis of DNA sequences, percentage divergence and genetic distances estimation (Clustal X, BioEdit, MEGA). Phylogenetic trees reconstruction using different methods including Maximum Likelihood and Bayesian Inference (MEGA, MrBayes). Molecular dating: divergence time estimations of the splitting events within species radiation by using relaxed and local molecular clock (MEGA, BEAST). Analysis of the genetic variability within and among populations (DnaSP, ARLEQUIN).

ITALY 2018